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| Gerard, Andre; Naciri, Yamama; Peignon, Jean-marie; Ledu, Christophe; Phelipot, Pascal; Noiret, Christophe; Peudenier, Isabelle; Grizel, Henri. |
| For determination of ploidy levels in bivalves, three techniques are now routinely used: karyological determination. microfluorimetry and flow cytometry. This paper proposes an alternative technique which is based on determination of the optical density (OD) of stained nuclei using image analysis. A karyological and image analysis comparative experiment conducted on juveniles of Crassostrea gigas (Thunberg) and Ruditapes philippinarum (Adam & Reeve) is described and highlights the advantages of the image analysis technique. Initially developed on C. gigas and R. philippinarum, the image analysis method was easily extended to other bivalve species and to different developmental stages, as demonstrated by two experiments conducted on Ostrea edulis (L.).... |
| Tipo: Text |
Palavras-chave: Estimation bivalve triploidy; Image analysis; Triploid; Genetic; Crassostrea gigas; Oysters. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/1994/publication-3200.pdf |
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